q Introduction

The production of recombinant proteins in suspension cell culture nowadays is a major issue in the biopharmaceutical industry. Each of the mammalian cell lines used for these process has individual characteristics, furthermore the different stages during the production process have different requirements. Process development and particularly media optimisation are of utmost importance to provide maximum cell numbers and highest protein yield.

The CultiFlask 50 disposable bioreactor is a versatile tool to perform media optimization | process development experiments in high numbers. It resembles the classic 50 ml centrifuge tube design, thus making handling steps like e.g. media exchange very convenient. Each bioreactor is equipped with a vented screw cap with five holes of different size for optimal gas exchange with the surrounding atmosphere; a PTFE membrane serves as a sterile barrier and minimizes liquid loss by evaporation. There is no stirrer or any other mixing mechanism extending into the bioreactor's interior; mass transfer is achieved by shaking in an appropriate incubationshaker (temperature, CO2 and humidity controlled; regulation of shaking amplitude and frequency). Due to the unsophisticated design the CultiFlask 50 disposable bioreactor provides an ideal tool to perform hundreds of parallel cell cultivations under concern of media optimization | process development experiments.

q Features and Benefits

- Centrifuge tube design- flexible working volume of 10-35 ml (generally about 40% of the total volume capacity); easy handling concerning e.g. media exchange or sampling, minimal expenses

Each tube represents an individual

- Bioreactor- Allows for parallel experiments in high numbers

- Passive mixing with no stirring mechanism inside- Low risk of contamination, less shear stress to the cells, negligible foaming

- Vented cap with PTFE membrane- Optimal gas exchange, reduced water
loss by evaporation

- Completely disposable- No need for elaborate cleaning or validation procedures, tubes are sacrificed after the run

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