Chromatography

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  Vivapure mini/maxi prepA/G
  Sartorius Stedim Biotech, µ¶ÀÏ

Vivapure mini/maxi prepA/G

 

 

 

 

 

Vivapure miniprepA/G are pre-packed centrifugal devices, which makes purification of monoclonal and polyclonal antibodies as easy as filtration.

The rapid 1-2-3 bind-wash-elute protocol especially lends itself to screening applications, where many samples are processed in parallel.

 

 

Protein A & G miniprep Kits

Centrifuge

Sample size

0.65 ml

Typical binding capacity

1 mg IgG/column

Number of re-uses

3

 

Protein A & G maxiprep Kits

Centrifuge1

Sample size

20 ml

Typical binding capacity

20 mg IgG/column

Number of re-uses

5

 

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Use Protein A or G Vivapure miniprep or maxiprep spin columns to purify antibodies from serum, ascites and tissue culture supernatant derived from static cultures or bioreactors:

 

- Purification of a wide range of monoclonal and polyclonal antibodies

- Rapid clean up antibodies for immuno-histochemical, immunofluorescence experiments or Western blots

- Removal of abundant IgG from human plasma for biomarker detection

- Titration of antibody yield during cell culture

 

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Device

Polypropylene

Resin

Protein A & G covalently coupled to agarose resin

 

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Device

Resin bed volume

Binding capacity per use

MiniprepA/G

0.23 ml

1 mg human IgG

MaxiprepA/G

1.6 ml

20 mg human IgG